BMRB Entry 11245

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11245
MolProbity Validation Chart

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Title:
Solution Structure of the murine ubiquitin-like 5 protein from RIKEN cDNA 0610031K06
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Hayashi, F.; Shirouzu, M.; Terada, T.; Kigawa, T.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Hirota, H.; Yoshida, M.; Tanaka, A.; Osanai, T.; Arakawa, T.; Carninci, P.; Kawai, J.; Hayashizaki, Y.; Yokoyama, S.
Citation:

Citation: Hayashi, F.; Shirouzu, M.; Terada, T.; Kigawa, T.; Inoue, M.; Yabuki, T.; Aoki, M.; Seki, E.; Matsuda, T.; Hirota, H.; Yoshida, M.; Tanaka, A.; Osanai, T.; Arakawa, T.; Carninci, P.; Kawai, J.; Hayashizaki, Y.; Yokoyama, S.. "Solution Structure of the murine ubiquitin-like 5 protein from RIKEN cDNA 0610031K06"  .

Assembly members:

Assembly members:
ubiquitin-like 5, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: 011010-5

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ubiquitin-like 5: MKGSSHHHHHHSSGASLVPR GSEGAATMIEVVCNDRLGKK VRVKCNTDDTIGDLKKLIAA QTGTRWNKIVLKKWYTIFKD HVSLGDYEIHDGMNLELYYQ

Data sets:
Data typeCount
13C chemical shifts415
15N chemical shifts99
1H chemical shifts662

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin-like 51

Entities:

Entity 1, ubiquitin-like 5 100 residues - Formula weight is not available

1   METLYSGLYSERSERHISHISHISHISHIS
2   HISSERSERGLYALASERLEUVALPROARG
3   GLYSERGLUGLYALAALATHRMETILEGLU
4   VALVALCYSASNASPARGLEUGLYLYSLYS
5   VALARGVALLYSCYSASNTHRASPASPTHR
6   ILEGLYASPLEULYSLYSLEUILEALAALA
7   GLNTHRGLYTHRARGTRPASNLYSILEVAL
8   LEULYSLYSTRPTYRTHRILEPHELYSASP
9   HISVALSERLEUGLYASPTYRGLUILEHIS
10   ASPGLYMETASNLEUGLULEUTYRTYRGLN

Samples:

sample_1: ubiquitin-like 5, [U-13C; U-15N], 0.5 mM; phosphate 20 mM; NaCl 500 mM; DTT 4 mM; NaN3 0.4 mM; H2O 92%; D2O 8%

condition_1: ionic strength: 520 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.816, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAB22312 BAB23111 BAB25215 BAB26545 BAB28481
EMBL CAH90527 CAQ34910 CBZ13168 CBZ13169 CCC41973
GB AAG34704 AAH07053 AAH28498 AAI02199 AAI10840
REF NP_001032799 NP_001041706 NP_001041708 NP_001125281 NP_001159534
SP Q3T0Z3 Q6EGX7 Q791B0 Q9BZL1 Q9EPV8
TPG DAA28014 DAA32797 DAA32925
AlphaFold Q3T0Z3 Q6EGX7 Q791B0 Q9BZL1 Q9EPV8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks