BMRB Entry 11236

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PDB ID: 2yr3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11236
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the fourth Ig-like domain from myosin light chain kinase, smooth muscle

Deposition date:

2010-07-23

Original release date:

2011-08-03

Authors:

Qin, X.; Kurosaki, C.; Yoshida, M.; Hayahsi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fourth Ig-like domain from myosin light chain kinase, smooth muscle" .

Assembly members:

Assembly members:
Ig domain, polymer, 99 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P061030-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ig domain: GSSGSSGMEVAPSFSSVLKD CAVIEGQDFVLQCSVRGTPV PRITWLLNGQPIQYARSTCE AGVAELHIQDALPEDHGTYT CLAENALGQVSCSAWVTVH

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	400
15N chemical shifts	96
1H chemical shifts	637

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ig domain	1

Entities:

Entity 1, Ig domain 99 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

GLU

VAL

2

ALA

PRO

SER

PHE

SER

VAL

LEU

LYS

ASP

3

CYS

ALA

VAL

ILE

GLU

GLY

GLN

ASP

PHE

VAL

4

LEU

GLN

CYS

SER

VAL

ARG

GLY

THR

PRO

VAL

5

PRO

ARG

ILE

THR

TRP

LEU

ASN

GLY

GLN

6

PRO

ILE

GLN

TYR

ALA

ARG

SER

THR

CYS

GLU

7

ALA

GLY

VAL

ALA

GLU

LEU

HIS

ILE

GLN

ASP

8

ALA

LEU

PRO

GLU

ASP

HIS

GLY

THR

TYR

THR

9

CYS

LEU

ALA

GLU

ASN

ALA

LEU

GLY

GLN

VAL

10

SER

CYS

SER

ALA

TRP

VAL

THR

VAL

HIS

Samples:

sample_1: Ig domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

Delta v4.3, JEOL - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

JEOL ECA 700 MHz