BMRB Entry 11234

Title:
Solution structure of the RING domain (1-66) from tripartite motif-containing protein 31
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the RING domain (1-66) from tripartite motif-containing protein 31"  .

Assembly members:

Assembly members:
RING-type zinc finger, polymer, 73 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060529-14

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts299
15N chemical shifts63
1H chemical shifts484

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RING-type zinc finger1
2ZINC ION no.12
3ZINC ION no.22

Entities:

Entity 1, RING-type zinc finger 73 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETALASER
2   GLYGLNPHEVALASNLYSLEUGLNGLUGLU
3   VALILECYSPROILECYSLEUASPILELEU
4   GLNLYSPROVALTHRILEASPCYSGLYHIS
5   ASNPHECYSLEULYSCYSILETHRGLNILE
6   GLYGLUTHRSERCYSGLYPHEPHELYSCYS
7   PROLEUCYSLYSTHRSERVALARGLYSASN
8   ALAILEARG

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: RING-type zinc finger, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAD96449 BAE78603 BAE92827 BAE92828 BAF82959
EMBL CAM26290
GB AAH17017 AAP35321 AAP36702 AAX41911 AAX41912
REF NP_008959 XP_003272070 XP_004043605 XP_008953353 XP_008953354
SP Q9BZY9
AlphaFold Q9BZY9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks