BMRB Entry 11232

Name: Solution structure of the PKD domain (329-428) from human KIAA0319
Published: 2011-08-03

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PDB ID: 2e7m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11232
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PKD domain (329-428) from human KIAA0319

Deposition date:

2010-07-23

Original release date:

2011-08-03

Authors:

Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PKD domain (329-428) from human KIAA0319" .

Assembly members:

Assembly members:
PKD domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P051121-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PKD domain: GSSGSSGPRTVKELTVSAGD NLIITLPDNEVELKAFVAPA PPVETTYNYEWNLISHPTDY QGEIKQGHKQTLNLSQLSVG LYVFKVTVSSENAFGEGFVN VTVKPARSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	475
15N chemical shifts	107
1H chemical shifts	750

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PKD domain	1

Entities:

Entity 1, PKD domain 113 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

ARG

THR

2

VAL

LYS

GLU

LEU

THR

VAL

SER

ALA

GLY

ASP

3

ASN

LEU

ILE

THR

LEU

PRO

ASP

ASN

GLU

4

VAL

GLU

LEU

LYS

ALA

PHE

VAL

ALA

PRO

ALA

5

PRO

VAL

GLU

THR

TYR

ASN

TYR

GLU

6

TRP

ASN

LEU

ILE

SER

HIS

PRO

THR

ASP

TYR

7

GLN

GLY

GLU

ILE

LYS

GLN

GLY

HIS

LYS

GLN

8

THR

LEU

ASN

LEU

SER

GLN

LEU

SER

VAL

GLY

9

LEU

TYR

VAL

PHE

LYS

VAL

THR

VAL

SER

10

GLU

ASN

ALA

PHE

GLY

GLU

GLY

PHE

VAL

ASN

11

VAL

THR

VAL

LYS

PRO

ALA

ARG

SER

GLY

PRO

12

SER

GLY

Samples:

sample_1: PKD domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2E7M
REF	XP_009448870 XP_011513329