BMRB Entry 11231

Name: Solution structure of the PKD domain from KIAA 1837 protein
Published: 2011-08-03

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PDB ID: 2yrl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11231
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PKD domain from KIAA 1837 protein

Deposition date:

2010-07-23

Original release date:

2011-08-03

Authors:

Qin, X.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PKD domain from KIAA 1837 protein" .

Assembly members:

Assembly members:
PKD domain, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P051025-02

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PKD domain: GSSGSSGQADAGPDKELTLP VDSTTLDGSKSSDDQKIISY LWEKTQGPDGVQLENANSSV ATVTGLQVGTYVFTLTVKDE RNLQSQSSVNVIVKEESGPS SG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	401
15N chemical shifts	98
1H chemical shifts	645

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PKD domain	1

Entities:

Entity 1, PKD domain 102 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLN

ALA

ASP

2

ALA

GLY

PRO

ASP

LYS

GLU

LEU

THR

LEU

PRO

3

VAL

ASP

SER

THR

LEU

ASP

GLY

SER

LYS

4

SER

ASP

GLN

LYS

ILE

SER

TYR

5

LEU

TRP

GLU

LYS

THR

GLN

GLY

PRO

ASP

GLY

6

VAL

GLN

LEU

GLU

ASN

ALA

ASN

SER

VAL

7

ALA

THR

VAL

THR

GLY

LEU

GLN

VAL

GLY

THR

8

TYR

VAL

PHE

THR

LEU

THR

VAL

LYS

ASP

GLU

9

ARG

ASN

LEU

GLN

SER

GLN

SER

VAL

ASN

10

VAL

ILE

VAL

LYS

GLU

SER

GLY

PRO

SER

11

SER

GLY

Samples:

sample_1: PKD domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 900 MHz