BMRB Entry 11226

Name: Solution structure of the PDZ domain from Human MAGUK p55 subfamily member 2
Published: 2011-08-03

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PDB ID: 2e7k
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11226
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PDZ domain from Human MAGUK p55 subfamily member 2

Deposition date:

2010-07-23

Original release date:

2011-08-03

Authors:

Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain from Human MAGUK p55 subfamily member 2" .

Assembly members:

Assembly members:
PDZ domain, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050912-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGRMVGIRKTAGEHL GVTFRVEGGELVIARILHGG MVAQQGLLHVGDIIKEVNGQ PVGSDPRALQELLRNASGSV ILKILSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	365
15N chemical shifts	87
1H chemical shifts	603

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 91 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

MET

VAL

2

GLY

ILE

ARG

LYS

THR

ALA

GLY

GLU

HIS

LEU

3

GLY

VAL

THR

PHE

ARG

VAL

GLU

GLY

GLU

4

LEU

VAL

ILE

ALA

ARG

ILE

LEU

HIS

GLY

5

MET

VAL

ALA

GLN

GLY

LEU

HIS

VAL

6

GLY

ASP

ILE

LYS

GLU

VAL

ASN

GLY

GLN

7

PRO

VAL

GLY

SER

ASP

PRO

ARG

ALA

LEU

GLN

8

GLU

LEU

ARG

ASN

ALA

SER

GLY

SER

VAL

9

ILE

LEU

LYS

ILE

LEU

SER

GLY

PRO

SER

10

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

Delta v4.3, JEOL - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

JEOL ECA 800 MHz

PDB	2E7K
DBJ	BAD97280 BAE24680 BAE36473 BAE42909 BAG57760
EMBL	CAA58067 CAB66489 CAI56746
GB	AAC78484 AAD44342 AAH30287 AAH53026 AAX29754
REF	NP_001180000 NP_001265299 NP_001265300 NP_001265301 NP_001265302
SP	Q14168 Q9WV34
TPG	DAA18383
AlphaFold	Q14168 Q9WV34