BMRB Entry 11221

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PDB ID: 2e6q
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11221
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Ig-like domain (615-713) from human Obscurin-like protein 1

Deposition date:

2010-07-22

Original release date:

2011-07-21

Authors:

Qin, X.; Suetake, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Suetake, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the Ig-like domain (615-713) from human Obscurin-like protein 1" .

Assembly members:

Assembly members:
Ig-like domain, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050725-17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ig-like domain: GSSGSSGTARLVAGLEDVQV YDGEDAVFSLDLSTIIQGTW FLNGEELKSNEPEGQVEPGA LRYRIEQKGLQHRLILHAVK HQDSGALVGFSCPGVQDSAA LTIQESSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	452
15N chemical shifts	112
1H chemical shifts	721

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Ig-like domain of Obscurin-like protein 1	1

Entities:

Entity 1, Ig-like domain of Obscurin-like protein 1 112 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

ALA

ARG

2

LEU

VAL

ALA

GLY

LEU

GLU

ASP

VAL

GLN

VAL

3

TYR

ASP

GLY

GLU

ASP

ALA

VAL

PHE

SER

LEU

4

ASP

LEU

SER

THR

ILE

GLN

GLY

THR

TRP

5

PHE

LEU

ASN

GLY

GLU

LEU

LYS

SER

ASN

6

GLU

PRO

GLU

GLY

GLN

VAL

GLU

PRO

GLY

ALA

7

LEU

ARG

TYR

ARG

ILE

GLU

GLN

LYS

GLY

LEU

8

GLN

HIS

ARG

LEU

ILE

LEU

HIS

ALA

VAL

LYS

9

HIS

GLN

ASP

SER

GLY

ALA

LEU

VAL

GLY

PHE

10

SER

CYS

PRO

GLY

VAL

GLN

ASP

SER

ALA

11

LEU

THR

ILE

GLN

GLU

SER

GLY

PRO

SER

12

SER

GLY

Samples:

sample_1: Ig-like domain, [U-13C; U-15N], 1.10 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz