BMRB Entry 11220

Name: Solution structure of the second SH3 domain of human KIAA0769 protein
Published: 2011-07-21

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PDB ID: 2dl7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11220
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the second SH3 domain of human KIAA0769 protein

Deposition date:

2010-07-22

Original release date:

2011-07-21

Authors:

Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Kurosaki, C.; Yoshida, M.; Hayashi, F.; Yokoyama, S.. "Solution structure of the second SH3 domain of human KIAA0769 protein" .

Assembly members:

Assembly members:
SH3 domain, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050725-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGVCFVKALYDYEGQ TDDELSFPEGAIIRILNKEN QDDDGFWEGEFNGRIGVFPS VLVEELSSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	297
15N chemical shifts	72
1H chemical shifts	454

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	second SH3 domain of human KIAA0769 protein	1

Entities:

Entity 1, second SH3 domain of human KIAA0769 protein 73 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

CYS

PHE

2

VAL

LYS

ALA

LEU

TYR

ASP

TYR

GLU

GLY

GLN

3

THR

ASP

GLU

LEU

SER

PHE

PRO

GLU

GLY

4

ALA

ILE

ARG

ILE

LEU

ASN

LYS

GLU

ASN

5

GLN

ASP

GLY

PHE

TRP

GLU

GLY

GLU

6

PHE

ASN

GLY

ARG

ILE

GLY

VAL

PHE

PRO

SER

7

VAL

LEU

VAL

GLU

LEU

SER

GLY

PRO

8

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.24 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

Delta v4.3, JEOL - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

JEOL ECA 800 MHz