BMRB Entry 11213

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11213
MolProbity Validation Chart

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Title:
Solution structure of the SH3 domain of Hypothetical protein SH3YL1
Deposition date:
2010-07-22
Original release date:
2011-07-21
Authors:
Qin, X.; Nagasima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Nagasima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the SH3 domain of Hypothetical protein SH3YL1"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050425-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGHERVGNLNQPIEV TALYSFEGQQPGDLNFQAGD RITVISKTDSHFDWWEGKLR GQTGIFPANYVTMNSGPSSG

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts80
1H chemical shifts500

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain of Hypothetical protein SH3YL11

Entities:

Entity 1, SH3 domain of Hypothetical protein SH3YL1 80 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYHISGLUARG
2   VALGLYASNLEUASNGLNPROILEGLUVAL
3   THRALALEUTYRSERPHEGLUGLYGLNGLN
4   PROGLYASPLEUASNPHEGLNALAGLYASP
5   ARGILETHRVALILESERLYSTHRASPSER
6   HISPHEASPTRPTRPGLUGLYLYSLEUARG
7   GLYGLNTHRGLYILEPHEPROALAASNTYR
8   VALTHRMETASNSERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.19 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAB15493 BAF85001 BAJ17777 BAK63510
EMBL CAB43679 CAG33642 CAH90143 CAH91158
GB AAH08374 AAH08375 AAY14957 ACE86416 ACE86417
REF NP_001125036 NP_001153069 NP_001238793 NP_001269611 NP_001269616
SP Q5RAQ2 Q96HL8
AlphaFold Q5RAQ2 Q96HL8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks