BMRB Entry 11212

Title:
Solution structure of the first ig-like domain of Myosin-binding protein C, slow-type
Deposition date:
2010-07-22
Original release date:
2011-07-21
Authors:
Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first ig-like domain of Myosin-binding protein C, slow-type"  .

Assembly members:

Assembly members:
IG domain, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050302-60

Data sets:
Data typeCount
13C chemical shifts550
15N chemical shifts114
1H chemical shifts865

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1IG domain of Myosin-binding protein C, slow-type1

Entities:

Entity 1, IG domain of Myosin-binding protein C, slow-type 126 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYILELEUPHE
2   ILEGLULYSPROGLNGLYGLYTHRVALLYS
3   VALGLYGLUASPILETHRPHEILEALALYS
4   VALLYSALAGLUASPLEULEUARGLYSPRO
5   THRILELYSTRPPHELYSGLYLYSTRPMET
6   ASPLEUALASERLYSALAGLYLYSHISLEU
7   GLNLEULYSGLUTHRPHEGLUARGHISSER
8   ARGVALTYRTHRPHEGLUMETGLNILEILE
9   LYSALALYSASPASNPHEALAGLYASNTYR
10   ARGCYSGLUVALTHRTYRLYSASPLYSPHE
11   ASPSERCYSSERPHEASPLEUGLUVALHIS
12   GLUSERTHRGLYTHRTHRPROASNILEASP
13   SERGLYPROSERSERGLY

Samples:

sample_1: IG domain, [U-13C; U-15N], 1.32 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

PDB
DBJ BAF85665 BAG51755 BAG59277 BAH13954
EMBL CAD38625 CAD38925 CAD89907 CAD89927 CAD91144
GB AAI43504 AAI43505 ACE86664 ACE87348 EAW97664
REF NP_001241647 NP_001241648 NP_001241649 NP_002456 NP_996555
SP Q00872
AlphaFold Q00872

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks