BMRB Entry 11206

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PDB ID: 1x5r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11206
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the fourth PDZ domain of Glutamate receptor interacting protein 2

Deposition date:

2010-07-22

Original release date:

2011-07-21

Authors:

Qin, X.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fourth PDZ domain of Glutamate receptor interacting protein 2" .

Assembly members:

Assembly members:
PDZ domain, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040705-16

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGGGQIVHTETTEVV LCGDPLSGFGLQLQGGIFAT ETLSSPPLVCFIEPDSPAER CGLLQVGDRVLSINGIATED GTMEEANQLLRDAALAHKVV LEVEFDSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	440
15N chemical shifts	104
1H chemical shifts	706

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain of Glutamate receptor interacting protein 2	1

Entities:

Entity 1, PDZ domain of Glutamate receptor interacting protein 2 112 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLN

2

ILE

VAL

HIS

THR

GLU

THR

GLU

VAL

3

LEU

CYS

GLY

ASP

PRO

LEU

SER

GLY

PHE

GLY

4

LEU

GLN

LEU

GLN

GLY

ILE

PHE

ALA

THR

5

GLU

THR

LEU

SER

PRO

LEU

VAL

CYS

6

PHE

ILE

GLU

PRO

ASP

SER

PRO

ALA

GLU

ARG

7

CYS

GLY

LEU

GLN

VAL

GLY

ASP

ARG

VAL

8

LEU

SER

ILE

ASN

GLY

ILE

ALA

THR

GLU

ASP

9

GLY

THR

MET

GLU

ALA

ASN

GLN

LEU

10

ARG

ASP

ALA

LEU

ALA

HIS

LYS

VAL

11

LEU

GLU

VAL

GLU

PHE

ASP

SER

GLY

PRO

SER

12

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 0.95 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, DelaglioF. - processing

NMRView v5.0.4, JohnsonB.A. - data analysis

Kujira v0.9296, KobayashiN. - data analysis

CYANA v1.0.7, GuntertP. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz
Varian INOVA 900 MHz