BMRB Entry 11205

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PDB ID: 2cot
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11205
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the first and second zf-C2H2 domain of Zinc finger protein 435

Deposition date:

2010-07-22

Original release date:

2011-07-21

Authors:

Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first and second zf-C2H2 domain of Zinc finger protein 435" .

Assembly members:

Assembly members:
ADK_lid domain, polymer, 77 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040628-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ADK_lid domain: GSSGSSGRSEWQQRERRRYK CDECGKSFSHSSDLSKHRRT HTGEKPYKCDECGKAFIQRS HLIGHHRVHTGSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	309
15N chemical shifts	66
1H chemical shifts	444

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ADK_lid domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, ADK_lid domain 77 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

SER

GLU

2

TRP

GLN

ARG

GLU

ARG

TYR

LYS

3

CYS

ASP

GLU

CYS

GLY

LYS

SER

PHE

SER

HIS

4

SER

ASP

LEU

SER

LYS

HIS

ARG

THR

5

HIS

THR

GLY

GLU

LYS

PRO

TYR

LYS

CYS

ASP

6

GLU

CYS

GLY

LYS

ALA

PHE

ILE

GLN

ARG

SER

7

HIS

LEU

ILE

GLY

HIS

ARG

VAL

HIS

THR

8

GLY

SER

GLY

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: ADK_lid domain, [U-13C; U-15N], 1.27 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.01 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, DelaglioF. - processing

NMRView v5.0.4, JohnsonB.A. - data analysis

Kujira v0.9296, KobayashiN. - data analysis

CYANA v2.0.17, GuntertP. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz