BMRB Entry 11204

Name: Solution structure of the second PDZ domain of harmonin protein
Published: 2011-07-21

Click here to enlarge.

PDB ID: 1x5n
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11204
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the second PDZ domain of harmonin protein

Deposition date:

2010-07-22

Original release date:

2011-07-21

Authors:

Qin, X.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the second PDZ domain of harmonin protein" .

Assembly members:

Assembly members:
PDZ domain, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: p040510-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGSPGNRENKEKKVF ISLVGSRGLGCSISSGPIQK PGIFISHVKPGSLSAEVGLE IGDQIVEVNGVDFSNLDHKE AVNVLKSSRSLTISIVAAAG RELFMTDRSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	463
15N chemical shifts	107
1H chemical shifts	738

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain of harmonin protein	1

Entities:

Entity 1, PDZ domain of harmonin protein 114 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

PRO

GLY

2

ASN

ARG

GLU

ASN

LYS

GLU

LYS

VAL

PHE

3

ILE

SER

LEU

VAL

GLY

SER

ARG

GLY

LEU

GLY

4

CYS

SER

ILE

SER

GLY

PRO

ILE

GLN

LYS

5

PRO

GLY

ILE

PHE

ILE

SER

HIS

VAL

LYS

PRO

6

GLY

SER

LEU

SER

ALA

GLU

VAL

GLY

LEU

GLU

7

ILE

GLY

ASP

GLN

ILE

VAL

GLU

VAL

ASN

GLY

8

VAL

ASP

PHE

SER

ASN

LEU

ASP

HIS

LYS

GLU

9

ALA

VAL

ASN

VAL

LEU

LYS

SER

ARG

SER

10

LEU

THR

ILE

SER

ILE

VAL

ALA

GLY

11

ARG

GLU

LEU

PHE

MET

THR

ASP

ARG

SER

GLY

12

PRO

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz