BMRB Entry 11202

Title:
Solution structure of the PDZ domain of Spinophilin/NeurabinII protein
Deposition date:
2010-07-22
Original release date:
2011-07-21
Authors:
Qin, X.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain of Spinophilin/NeurabinII protein"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P021030-40

Experimental source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P021030-40

Data sets:
Data typeCount
13C chemical shifts433
15N chemical shifts113
1H chemical shifts711

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 107 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEUGLULEU
2   PHEPROVALGLULEUGLULYSASPGLUASP
3   GLYLEUGLYILESERILEILEGLYMETGLY
4   VALGLYALAASPALAGLYLEUGLULYSLEU
5   GLYILEPHEVALLYSTHRVALTHRGLUGLY
6   GLYALAALAGLNARGASPGLYARGILEGLN
7   VALASNASPGLNILEVALGLUVALASPGLY
8   ILESERLEUVALGLYVALTHRGLNASNPHE
9   ALAALATHRVALLEUARGASNTHRLYSGLY
10   ASNVALARGPHEVALILEGLYARGGLULYS
11   PROSERGLYPROSERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.05 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio.F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA86536 BAC77245 BAE22449 BAE23620
GB AAC53454 AAH53748 AAI18112 AAI30450 AAI44110
REF NP_001069621 NP_001159632 NP_001159633 NP_001159634 NP_001159635
SP O35867 Q9ULJ8
TPG DAA30807
AlphaFold Q9ULJ8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks