BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11201

Title: Solution structure of the fourth PDZ domain of KIAA1095 protein

Deposition date: 2010-07-22 Original release date: 2011-07-21

Authors: Qin, X.; Saito, K.; Kigawa, T.; Hayashi, F.; Yokoyama, S.

Citation: Qin, X.; Saito, K.; Kigawa, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fourth PDZ domain of KIAA1095 protein"  .

Assembly members:
PDZ domain, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis' 'E. coli - cell free' 'E. coli

Entity Sequences (FASTA):
PDZ domain: GSSGSSGDIHQEMDREELEL EEVDLYRMNSQDKLGLTVCY RTDDEDDIGIYISEIDPNSI AAKDGRIREGDRIIQINGIE VQNREEAVALLTSEENKNFS LLIARPELQLDEGWMDDDSG PSSG

Data sets:
Data typeCount
13C chemical shifts519
15N chemical shifts139
1H chemical shifts844

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain of KIAA1095 protein1

Entities:

Entity 1, PDZ domain of KIAA1095 protein 124 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPILEHIS
2   GLNGLUMETASPARGGLUGLULEUGLULEU
3   GLUGLUVALASPLEUTYRARGMETASNSER
4   GLNASPLYSLEUGLYLEUTHRVALCYSTYR
5   ARGTHRASPASPGLUASPASPILEGLYILE
6   TYRILESERGLUILEASPPROASNSERILE
7   ALAALALYSASPGLYARGILEARGGLUGLY
8   ASPARGILEILEGLNILEASNGLYILEGLU
9   VALGLNASNARGGLUGLUALAVALALALEU
10   LEUTHRSERGLUGLUASNLYSASNPHESER
11   LEULEUILEALAARGPROGLULEUGLNLEU
12   ASPGLUGLYTRPMETASPASPASPSERGLY
13   PROSERSERGLY

Samples:

sample_1: PDZ domain' '[U-13C; U-15N]; natural abundance; natural abundance; natural abundance; natural abundance; .; .

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio.F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA83047 BAC11068 BAD32376 BAE25317 BAG09968
GB AAF22131 AAF22132 AAH10329 AAI50624 AAI52418
REF NP_001258180 NP_001290068 NP_001290069 NP_001290070 NP_001290071
SP P68907 Q69ZS0 Q9UPQ7
TPG DAA17105 DAA17106

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts