BMRB Entry 11199

Name: Solution structure of the PDZ domain of Enigma homologue protein
Published: 2011-07-21

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PDB ID: 1wf7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11199
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PDZ domain of Enigma homologue protein

Deposition date:

2010-07-22

Original release date:

2011-07-21

Authors:

Qin, X.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Qin, X.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain of Enigma homologue protein" .

Assembly members:

Assembly members:
PDZ domain, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P030203-44

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGSVSLVGPAPWGFR LQGGKDFNMPLTISSLKDGG KASQAHVRIGDVVLSIDGIS AQGMTHLEAQNKIKACTGSL NMTLQRASAAAKSEPVSSGP SSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	407
15N chemical shifts	106
1H chemical shifts	657

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Enigma homologue protein	1

Entities:

Entity 1, Enigma homologue protein 103 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

SER

VAL

SER

2

LEU

VAL

GLY

PRO

ALA

PRO

TRP

GLY

PHE

ARG

3

LEU

GLN

GLY

LYS

ASP

PHE

ASN

MET

PRO

4

LEU

THR

ILE

SER

LEU

LYS

ASP

GLY

5

LYS

ALA

SER

GLN

ALA

HIS

VAL

ARG

ILE

GLY

6

ASP

VAL

LEU

SER

ILE

ASP

GLY

ILE

SER

7

ALA

GLN

GLY

MET

THR

HIS

LEU

GLU

ALA

GLN

8

ASN

LYS

ILE

LYS

ALA

CYS

THR

GLY

SER

LEU

9

ASN

MET

THR

LEU

GLN

ARG

ALA

SER

ALA

10

ALA

LYS

SER

GLU

PRO

VAL

SER

GLY

PRO

11

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.06 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio.F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1WF7
DBJ	BAA88828 BAB22633 BAB26304 BAE28279
GB	ABB03726 ADI88504 ADI88505 ADI88506 ADI88507
REF	NP_001177781 NP_001177782 NP_001177783 NP_001177784 NP_001177785
SP	Q8CI51
AlphaFold	Q8CI51