BMRB Entry 11195

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PDB ID: 2dls
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11195
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PDZ domain of human Rho guanine nucleotide exchange factor 11

Deposition date:

2010-07-21

Original release date:

2011-07-20

Authors:

Inoue, K.; Suetake, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Inoue, K.; Suetake, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain of human Rho guanine nucleotide exchange factor 11" .

Assembly members:

Assembly members:
PDZ domain, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050919-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGVQRCVIIQKDQHG FGFTVSGDRIVLVQSVRPGG AAMKAGVKEGDRIIKVNGTM VTNSSHLEVVKLIKSGAYVA LTLLGSSSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	357
15N chemical shifts	83
1H chemical shifts	588

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 93 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

VAL

GLN

ARG

2

CYS

VAL

ILE

GLN

LYS

ASP

GLN

HIS

GLY

3

PHE

GLY

PHE

THR

VAL

SER

GLY

ASP

ARG

ILE

4

VAL

LEU

VAL

GLN

SER

VAL

ARG

PRO

GLY

5

ALA

MET

LYS

ALA

GLY

VAL

LYS

GLU

GLY

6

ASP

ARG

ILE

LYS

VAL

ASN

GLY

THR

MET

7

VAL

THR

ASN

SER

HIS

LEU

GLU

VAL

8

LYS

LEU

ILE

LYS

SER

GLY

ALA

TYR

VAL

ALA

9

LEU

THR

LEU

GLY

SER

GLY

PRO

10

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.20 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.93191, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2DLS
GB	AAH57394 EAW52892 EHH15364 EHH50383
REF	NP_937879 XP_001116843 XP_001167782 XP_002810045 XP_003937918