BMRB Entry 11191

Name: Solution structure of the eighth PDZ domain of human InaD-like protein
Published: 2011-07-20

Click here to enlarge.

PDB ID: 2dm8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11191
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Solution structure of the eighth PDZ domain of human InaD-like protein

Deposition date:

2010-07-21

Original release date:

2011-07-20

Authors:

Inoue, K.; Nagashima, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Inoue, K.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the eighth PDZ domain of human InaD-like protein" .

Assembly members:

Assembly members:
PDZ domain, polymer, 116 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050627-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGPATCPIVPGQEMI IEISKGRSGLGLSIVGGKDT PLNAIVIHEVYEEGAAARDG RLWAGDQILEVNGVDLRNSS HEEAITALRQTPQKVRLVVY RDEAHYRDEESGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	458
15N chemical shifts	106
1H chemical shifts	746

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 116 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

ALA

THR

2

CYS

PRO

ILE

VAL

PRO

GLY

GLN

GLU

MET

ILE

3

ILE

GLU

ILE

SER

LYS

GLY

ARG

SER

GLY

LEU

4

GLY

LEU

SER

ILE

VAL

GLY

LYS

ASP

THR

5

PRO

LEU

ASN

ALA

ILE

VAL

ILE

HIS

GLU

VAL

6

TYR

GLU

GLY

ALA

ARG

ASP

GLY

7

ARG

LEU

TRP

ALA

GLY

ASP

GLN

ILE

LEU

GLU

8

VAL

ASN

GLY

VAL

ASP

LEU

ARG

ASN

SER

9

HIS

GLU

ALA

ILE

THR

ALA

LEU

ARG

GLN

10

THR

PRO

GLN

LYS

VAL

ARG

LEU

VAL

TYR

11

ARG

ASP

GLU

ALA

HIS

TYR

ARG

ASP

GLU

12

SER

GLY

PRO

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.93191, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz