BMRB Entry 11190

Title:
Solution structure of the third PDZ domain of human InaD-like protein
Deposition date:
2010-07-21
Original release date:
2011-07-20
Authors:
Inoue, K.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Inoue, K.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the third PDZ domain of human InaD-like protein"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050627-17

Data sets:
Data typeCount
13C chemical shifts509
15N chemical shifts123
1H chemical shifts812

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 129 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYSERASP
2   SERSERLEUPHEGLUTHRTYRASNVALGLU
3   LEUVALARGLYSASPGLYGLNSERLEUGLY
4   ILEARGILEVALGLYTYRVALGLYTHRSER
5   HISTHRGLYGLUALASERGLYILETYRVAL
6   LYSSERVALILEPROGLYSERALAALATYR
7   HISASNGLYHISILEGLNVALASNASPLYS
8   ILEVALALAVALASPGLYVALASNILEGLN
9   GLYPHEALAASNHISASPVALVALGLUVAL
10   LEUARGASNALAGLYGLNVALVALHISLEU
11   THRLEUVALARGARGLYSTHRSERSERSER
12   THRSERPROLEUGLUPROPROSERASPARG
13   GLYTHRVALSERGLYPROSERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.26 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.93191, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks