BMRB Entry 11187

Name: Solution structure of the 7th PDZ domain of InaD-like protein
Published: 2011-07-20

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PDB ID: 2daz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11187
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 7th PDZ domain of InaD-like protein

Deposition date:

2010-07-21

Original release date:

2011-07-20

Authors:

Inoue, K.; Nagashima, T.; Izumi, K.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Inoue, K.; Nagashima, T.; Izumi, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the 7th PDZ domain of InaD-like protein" .

Assembly members:

Assembly members:
PDZ domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050302-33

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGDAFTDQKIRQRYA DLPGELHIIELEKDKNGLGL SLAGNKDRSRMSIFVVGINP EGPAAADGRMRIGDELLEIN NQILYGRSHQNASAIIKTAP SKVKLVFIRNEDAVNQMASG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	499
15N chemical shifts	121
1H chemical shifts	818

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 124 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

ALA

PHE

2

THR

ASP

GLN

LYS

ILE

ARG

GLN

ARG

TYR

ALA

3

ASP

LEU

PRO

GLY

GLU

LEU

HIS

ILE

GLU

4

LEU

GLU

LYS

ASP

LYS

ASN

GLY

LEU

GLY

LEU

5

SER

LEU

ALA

GLY

ASN

LYS

ASP

ARG

SER

ARG

6

MET

SER

ILE

PHE

VAL

GLY

ILE

ASN

PRO

7

GLU

GLY

PRO

ALA

ASP

GLY

ARG

MET

8

ARG

ILE

GLY

ASP

GLU

LEU

GLU

ILE

ASN

9

ASN

GLN

ILE

LEU

TYR

GLY

ARG

SER

HIS

GLN

10

ASN

ALA

SER

ALA

ILE

LYS

THR

ALA

PRO

11

SER

LYS

VAL

LYS

LEU

VAL

PHE

ILE

ARG

ASN

12

GLU

ASP

ALA

VAL

ASN

GLN

MET

ALA

SER

GLY

13

PRO

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.93191, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2DAZ
REF	XP_003361134 XP_008592429