BMRB Entry 11186

Name: Solution structure of the fifth PDZ domain of InaD-like protein
Published: 2011-07-20

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PDB ID: 2d92
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11186
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the fifth PDZ domain of InaD-like protein

Deposition date:

2010-07-21

Original release date:

2011-07-20

Authors:

Inoue, K.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Inoue, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fifth PDZ domain of InaD-like protein" .

Assembly members:

Assembly members:
PDZ domain, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050214-02

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGELALWSPEVKIVE LVKDCKGLGFSILDYQDPLD PTRSVIVIRSLVADGVAERS GGLLPGDRLVSVNEYCLDNT SLAEAVEILKAVPPGLVHLG ICSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	445
15N chemical shifts	99
1H chemical shifts	721

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PDZ domain	1

Entities:

Entity 1, PDZ domain 108 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

LEU

ALA

2

LEU

TRP

SER

PRO

GLU

VAL

LYS

ILE

VAL

GLU

3

LEU

VAL

LYS

ASP

CYS

LYS

GLY

LEU

GLY

PHE

4

SER

ILE

LEU

ASP

TYR

GLN

ASP

PRO

LEU

ASP

5

PRO

THR

ARG

SER

VAL

ILE

VAL

ILE

ARG

SER

6

LEU

VAL

ALA

ASP

GLY

VAL

ALA

GLU

ARG

SER

7

GLY

LEU

PRO

GLY

ASP

ARG

LEU

VAL

8

SER

VAL

ASN

GLU

TYR

CYS

LEU

ASP

ASN

THR

9

SER

LEU

ALA

GLU

ALA

VAL

GLU

ILE

LEU

LYS

10

ALA

VAL

PRO

GLY

LEU

VAL

HIS

LEU

GLY

11

ILE

CYS

SER

GLY

PRO

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.43 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.93191, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz