BMRB Entry 11185

Title:
Solution structure of the third SH3 domain of human RIM-binding protein 2
Deposition date:
2010-07-21
Original release date:
2011-07-20
Authors:
Inoue, K.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Inoue, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the third SH3 domain of human RIM-binding protein 2"  .

Assembly members:

Assembly members:
SH3 domain, polymer, 76 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P040705-06

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts69
1H chemical shifts456

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 76 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGARGMET
2   VALALALEUTYRASPTYRASPPROARGGLU
3   SERSERPROASNVALASPVALGLUALAGLU
4   LEUTHRPHECYSTHRGLYASPILEILETHR
5   VALPHEGLYGLUILEASPGLUASPGLYPHE
6   TYRTYRGLYGLULEUASNGLYGLNLYSGLY
7   LEUVALPROSERASNPHELEUGLUGLUVAL
8   SERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.08 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks