BMRB Entry 11184

Name: Solution structure of the second SH3 domain of human RIM-binding protein 2
Published: 2011-07-20

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PDB ID: 2csq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11184
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the second SH3 domain of human RIM-binding protein 2

Deposition date:

2010-07-21

Original release date:

2011-07-20

Authors:

Inoue, K.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Inoue, K.; Hayashi, F.; Yokoyama, S.. "Solution structure of the second SH3 domain of human RIM-binding protein 2" .

Assembly members:

Assembly members:
SH3 domain, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040705-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 domain: GSSGSSGTDPGAEELPARIF VALFDYDPLTMSPNPDAAEE ELPFKEGQIIKVYGDKDADG FYRGETCARLGLIPCNMVSE IQADDEEMMDQSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	378
15N chemical shifts	86
1H chemical shifts	583

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SH3 domain	1

Entities:

Entity 1, SH3 domain 97 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

ASP

PRO

2

GLY

ALA

GLU

LEU

PRO

ALA

ARG

ILE

PHE

3

VAL

ALA

LEU

PHE

ASP

TYR

ASP

PRO

LEU

THR

4

MET

SER

PRO

ASN

PRO

ASP

ALA

GLU

5

GLU

LEU

PRO

PHE

LYS

GLU

GLY

GLN

ILE

6

LYS

VAL

TYR

GLY

ASP

LYS

ASP

ALA

ASP

GLY

7

PHE

TYR

ARG

GLY

GLU

THR

CYS

ALA

ARG

LEU

8

GLY

LEU

ILE

PRO

CYS

ASN

MET

VAL

SER

GLU

9

ILE

GLN

ALA

ASP

GLU

MET

ASP

10

GLN

SER

GLY

PRO

SER

GLY

Samples:

sample_1: SH3 domain, [U-13C; U-15N], 1.01 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9296, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	2CSQ
GB	EPY74387
REF	XP_014422001