BMRB Entry 11181

Name: Solution structure of the fourth fn3 domain of KIAA1496 protein
Published: 2011-07-20

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PDB ID: 1wj3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11181
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the fourth fn3 domain of KIAA1496 protein

Deposition date:

2010-07-21

Original release date:

2011-07-20

Authors:

Inoue, K.; Nagashima, T.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Inoue, K.; Nagashima, T.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the fourth fn3 domain of KIAA1496 protein" .

Assembly members:

Assembly members:
fn3 domain, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P030728-76

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fn3 domain: GSSGSSGTVNVTTKKTPPSQ PPGNVVWNATDTKVLLNWEQ VKAMENESEVTGYKVFYRTS SQNNVQVLNTNKTSAELVLP IKEDYIIEVKATTDGGDGTS SEQIRIPRITSSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	473
15N chemical shifts	112
1H chemical shifts	767

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	fn3 domain	1

Entities:

Entity 1, fn3 domain 117 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

THR

VAL

ASN

2

VAL

THR

LYS

THR

PRO

SER

GLN

3

PRO

GLY

ASN

VAL

TRP

ASN

ALA

THR

4

ASP

THR

LYS

VAL

LEU

ASN

TRP

GLU

GLN

5

VAL

LYS

ALA

MET

GLU

ASN

GLU

SER

GLU

VAL

6

THR

GLY

TYR

LYS

VAL

PHE

TYR

ARG

THR

SER

7

SER

GLN

ASN

VAL

GLN

VAL

LEU

ASN

THR

8

ASN

LYS

THR

SER

ALA

GLU

LEU

VAL

LEU

PRO

9

ILE

LYS

GLU

ASP

TYR

ILE

GLU

VAL

LYS

10

ALA

THR

ASP

GLY

ASP

GLY

THR

SER

11

SER

GLU

GLN

ILE

ARG

ILE

PRO

ARG

ILE

THR

12

SER

GLY

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 0.98 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1WJ3
GB	EHH51179
REF	XP_005196486