BMRB Entry 11175

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11175
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Solution structure of calponin homology domain of IQGAP1
Deposition date:
2010-06-09
Original release date:
2010-09-09
Authors:
Umemoto, Ryo; Nishida, Noritaka; Ogino, Shinji; Shimada, Ichio
Citation:

Citation: Umemoto, Ryo; Nishida, Noritaka; Ogino, Shinji; Shimada, Ichio. "NMR structure of the calponin homology domain of human IQGAP1 and its implications for the actin recognition mode"  J. Biomol. NMR 48, 59-64 (2010).
PubMed: 20644981

Assembly members:

Assembly members:
CH domain, polymer, 190 residues, 22032.408 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6p

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GPLGSLTAEEMDERRRQNVA YEYLCHLEEAKRWMEACLGE DLPPTTELEEGLRNGVYLAK LGNFFSPKVVSLKKIYDREQ TRYKATGLHFRHTDNVIQWL NAMDEIGLPKIFYPETTDIY DRKNMPRCIYCIHALSLYLF KLGLAPQIQDLYGKVDFTEE EINNMKTELEKYGIQMPAFS KIGGILANEL

Data sets:
Data typeCount
13C chemical shifts651
15N chemical shifts180
1H chemical shifts1262

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CH domain1

Entities:

Entity 1, CH domain 190 residues - 22032.408 Da.

1   GLYPROLEUGLYSERLEUTHRALAGLUGLU
2   METASPGLUARGARGARGGLNASNVALALA
3   TYRGLUTYRLEUCYSHISLEUGLUGLUALA
4   LYSARGTRPMETGLUALACYSLEUGLYGLU
5   ASPLEUPROPROTHRTHRGLULEUGLUGLU
6   GLYLEUARGASNGLYVALTYRLEUALALYS
7   LEUGLYASNPHEPHESERPROLYSVALVAL
8   SERLEULYSLYSILETYRASPARGGLUGLN
9   THRARGTYRLYSALATHRGLYLEUHISPHE
10   ARGHISTHRASPASNVALILEGLNTRPLEU
11   ASNALAMETASPGLUILEGLYLEUPROLYS
12   ILEPHETYRPROGLUTHRTHRASPILETYR
13   ASPARGLYSASNMETPROARGCYSILETYR
14   CYSILEHISALALEUSERLEUTYRLEUPHE
15   LYSLEUGLYLEUALAPROGLNILEGLNASP
16   LEUTYRGLYLYSVALASPPHETHRGLUGLU
17   GLUILEASNASNMETLYSTHRGLULEUGLU
18   LYSTYRGLYILEGLNMETPROALAPHESER
19   LYSILEGLYGLYILELEUALAASNGLULEU

Samples:

sample_1: CH domain, [U-99% 13C; U-99% 15N], 0.90 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

sample_2: CH domain, [U-99% 13C; U-99% 15N], 0.90 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 1 mM; D2O 100%

sample_3: CH domain, [U-99% 15N], 0.90 mM; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; sodium azide 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.17 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D HCCH-COSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNHAsample_3isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA06123 BAB30486 BAC26450 BAC97854 BAE37174
EMBL CAH89415
GB AAA59187 AAF60344 AAH05906 AAH20549 AAH22105
REF NP_001101959 NP_003861 NP_057930 XP_001364413 XP_002804991
SP P46940 Q9JKF1
TPG DAA17679
AlphaFold P46940 Q9JKF1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks