BMRB Entry 11164

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PDB ID: 2ecy
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11164
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of TNF receptor-associated factor 3

Deposition date:

2010-04-15

Original release date:

2011-05-05

Authors:

Abe, H.; Miyamoto, K.; Tochio, N.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Abe, H.; Miyamoto, K.; Tochio, N.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of TNF receptor-associated factor 3" .

Assembly members:

Assembly members:
RING-type, residues 8-66, polymer, 66 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060515-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING-type, residues 8-66: GSSGSSGFVKTVEDKYKCEK CHLVLCSPKQTECGHRFCES CMAALLSSSSPKCTACQESI VKDKVF

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	270
15N chemical shifts	59
1H chemical shifts	413

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING-type, residues 8-66	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING-type, residues 8-66 66 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PHE

VAL

LYS

2

THR

VAL

GLU

ASP

LYS

TYR

LYS

CYS

GLU

LYS

3

CYS

HIS

LEU

VAL

LEU

CYS

SER

PRO

LYS

GLN

4

THR

GLU

CYS

GLY

HIS

ARG

PHE

CYS

GLU

SER

5

CYS

MET

ALA

LEU

SER

6

PRO

LYS

CYS

THR

ALA

CYS

GLN

GLU

SER

ILE

7

VAL

LYS

ASP

LYS

VAL

PHE

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING-type, residues 8-66, [U-13C; U-15N], 1.10 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2ECY
DBJ	BAH13910 BAI45792
EMBL	CAD62311
GB	AAA56753 AAA65732 AAA68195 AAC50112 AAH75086
REF	NP_001186356 NP_003291 NP_663777 NP_663778 XP_004055781
SP	Q13114
AlphaFold	Q13114