BMRB Entry 11163

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PDB ID: 2ecv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11163
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of Tripartite motif-containing protein 5

Deposition date:

2010-04-15

Original release date:

2011-05-05

Authors:

Abe, H.; Miyamoto, K.; Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Abe, H.; Miyamoto, K.; Tochio, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Zinc finger, C3HC4 type (RING finger) domain of Tripartite motif-containing protein 5" .

Assembly members:

Assembly members:
Zinc finger, C3HC4 type (RING finger), polymer, 85 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060411-09

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Zinc finger, C3HC4 type (RING finger): GSSGSSGMASGILVNVKEEV TCPICLELLTQPLSLDCGHS FCQACLTANHKKSMLDKGES SCPVCRISYQPENIRPNRHV ANIVE

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	337
15N chemical shifts	73
1H chemical shifts	551

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Zinc finger, C3HC4 type (RING finger)	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, Zinc finger, C3HC4 type (RING finger) 85 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

ALA

SER

2

GLY

ILE

LEU

VAL

ASN

VAL

LYS

GLU

VAL

3

THR

CYS

PRO

ILE

CYS

LEU

GLU

LEU

THR

4

GLN

PRO

LEU

SER

LEU

ASP

CYS

GLY

HIS

SER

5

PHE

CYS

GLN

ALA

CYS

LEU

THR

ALA

ASN

HIS

6

LYS

SER

MET

LEU

ASP

LYS

GLY

GLU

SER

7

SER

CYS

PRO

VAL

CYS

ARG

ILE

SER

TYR

GLN

8

PRO

GLU

ASN

ILE

ARG

PRO

ASN

ARG

HIS

VAL

9

ALA

ASN

ILE

VAL

GLU

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Zinc finger, C3HC4 type (RING finger), [U-13C; U-15N], 1.25 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: . K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2ECV
DBJ	BAB55218 BAG16811 BAK63054
GB	AAG53479 AAG53480 AAG53481 AAG53482 AAG53483
REF	NP_001012668 NP_001266478 NP_149023 NP_149083 NP_149084
SP	Q1ACD8 Q5C8T6 Q5D7J1 Q9C035
AlphaFold	Q1ACD8 Q5C8T6 Q5D7J1 Q9C035