BMRB Entry 11161

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PDB ID: 2ecw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11161
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Zinc finger, C3HC4 type (RING finger) domain Tripartite motif protein 30

Deposition date:

2010-04-15

Original release date:

2011-05-05

Authors:

Abe, H.; Miyamoto, K.; Tochio, N.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Abe, H.; Miyamoto, K.; Tochio, N.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the Zinc finger, C3HC4 type (RING finger) domain Tripartite motif protein 30" .

Assembly members:

Assembly members:
RING-type, residues 6-85, polymer, 85 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060417-23

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RING-type, residues 6-85: GSSGSSGMASSVLEMIKEEV TCPICLELLKEPVSADCNHS FCRACITLNYESNRNTDGKG NCPVCRVPYPFGNLKPNLHV ANIVE

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	347
15N chemical shifts	81
1H chemical shifts	553

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RING-type, residues 6-85	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, RING-type, residues 6-85 85 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

ALA

SER

2

SER

VAL

LEU

GLU

MET

ILE

LYS

GLU

VAL

3

THR

CYS

PRO

ILE

CYS

LEU

GLU

LEU

LYS

4

GLU

PRO

VAL

SER

ALA

ASP

CYS

ASN

HIS

SER

5

PHE

CYS

ARG

ALA

CYS

ILE

THR

LEU

ASN

TYR

6

GLU

SER

ASN

ARG

ASN

THR

ASP

GLY

LYS

GLY

7

ASN

CYS

PRO

VAL

CYS

ARG

VAL

PRO

TYR

PRO

8

PHE

GLY

ASN

LEU

LYS

PRO

ASN

LEU

HIS

VAL

9

ALA

ASN

ILE

VAL

GLU

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RING-type, residues 6-85, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2ECW
DBJ	BAE22032 BAE23387 BAE23595
GB	AAA40073 AAG53468 AAG53469 AAH05447 EDL16723
REF	NP_033125 XP_006507534
SP	P15533
AlphaFold	P15533