BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11155

Title: Solution structure of the SH3 domain of Abl interactor 2 (Abelson interactor 2)

Deposition date: 2010-04-15 Original release date: 2011-05-05

Authors: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Kigawa, T.; Yokoyama, S.

Citation: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of Abl interactor 2 (Abelson interactor 2)"  .

Assembly members:
SH3 domain, polymer, 78 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis' 'E. coli - cell free

Entity Sequences (FASTA):
SH3 domain: GSSGSSGDPPWAPRSYLEKV VAIYDYTKDKEDELSFQEGA IIYVIKKNDDGWYEGVMNGV TGLFPGNYVESISGPSSG

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts70
1H chemical shifts491

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 domain1

Entities:

Entity 1, SH3 domain 78 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPPROPRO
2   TRPALAPROARGSERTYRLEUGLULYSVAL
3   VALALAILETYRASPTYRTHRLYSASPLYS
4   GLUASPGLULEUSERPHEGLNGLUGLYALA
5   ILEILETYRVALILELYSLYSASNASPASP
6   GLYTRPTYRGLUGLYVALMETASNGLYVAL
7   THRGLYLEUPHEPROGLYASNTYRVALGLU
8   SERILESERGLYPROSERSERGLY

Samples:

sample_1: SH3 domain' '[U-13C; U-15N]; natural abundance; natural abundance; natural abundance; natural abundance; .; .

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAE23233 BAE89457 BAG57674 BAG59937 BAG61587
EMBL CAA64885 CAA64980 CAH89983 CAJ82503
GB AAA75446 AAA92289 AAC53493 AAF70308 AAH01439
REF NP_001016044 NP_001094641 NP_001124937 NP_001185499 NP_001185500
SP P62484 Q9NYB9
TPG DAA32557

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts