BMRB Entry 11155
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11155
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of Abl interactor 2 (Abelson interactor 2)" .
Assembly members:
SH3 domain, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050704-12
Entity Sequences (FASTA):
SH3 domain: GSSGSSGDPPWAPRSYLEKV
VAIYDYTKDKEDELSFQEGA
IIYVIKKNDDGWYEGVMNGV
TGLFPGNYVESISGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 322 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 491 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 78 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASP | PRO | PRO | ||||
| 2 | TRP | ALA | PRO | ARG | SER | TYR | LEU | GLU | LYS | VAL | ||||
| 3 | VAL | ALA | ILE | TYR | ASP | TYR | THR | LYS | ASP | LYS | ||||
| 4 | GLU | ASP | GLU | LEU | SER | PHE | GLN | GLU | GLY | ALA | ||||
| 5 | ILE | ILE | TYR | VAL | ILE | LYS | LYS | ASN | ASP | ASP | ||||
| 6 | GLY | TRP | TYR | GLU | GLY | VAL | MET | ASN | GLY | VAL | ||||
| 7 | THR | GLY | LEU | PHE | PRO | GLY | ASN | TYR | VAL | GLU | ||||
| 8 | SER | ILE | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 0.73 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE23233 BAE89457 BAG57674 BAG59937 BAG61587 |
| EMBL | CAA64885 CAA64980 CAH89983 CAJ82503 |
| GB | AAA75446 AAA92289 AAC53493 AAF70308 AAH01439 |
| REF | NP_001016044 NP_001094641 NP_001124937 NP_001185499 NP_001185500 |
| SP | P62484 Q9NYB9 |
| TPG | DAA32557 |
| AlphaFold | P62484 Q9NYB9 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks