BMRB Entry 11153
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11153
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Abe, H.; Tochio, N.; Miyamoto, K.; Saito, K.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SH3 domain of 130 kDa phosphatidylinositol
4,5-biphosphate-dependent ARF1 GTPase-activating protein" .
Assembly members:
SH3 domain, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050627-26
Entity Sequences (FASTA):
SH3 domain: GSSGSSGNKVRRVKTIYDCQ
ADNDDELTFIEGEVIIVTGE
EDQEWWIGHIEGQPERKGVF
PVSFVHILSDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 296 |
| 15N chemical shifts | 69 |
| 1H chemical shifts | 468 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 76 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | LYS | VAL | ||||
| 2 | ARG | ARG | VAL | LYS | THR | ILE | TYR | ASP | CYS | GLN | ||||
| 3 | ALA | ASP | ASN | ASP | ASP | GLU | LEU | THR | PHE | ILE | ||||
| 4 | GLU | GLY | GLU | VAL | ILE | ILE | VAL | THR | GLY | GLU | ||||
| 5 | GLU | ASP | GLN | GLU | TRP | TRP | ILE | GLY | HIS | ILE | ||||
| 6 | GLU | GLY | GLN | PRO | GLU | ARG | LYS | GLY | VAL | PHE | ||||
| 7 | PRO | VAL | SER | PHE | VAL | HIS | ILE | LEU | SER | ASP | ||||
| 8 | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.15 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9747, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 11081 11082 |
| PDB | |
| DBJ | BAA86563 BAC65759 BAE27250 BAE34783 |
| EMBL | CAD97831 |
| GB | AAB82338 AAC98349 AAC98350 AAH02201 AAH48818 |
| REF | NP_001037710 NP_001234925 NP_001263390 NP_001263391 NP_001263392 |
| SP | Q1AAU6 Q9QWY8 Q9ULH1 |
| AlphaFold | Q1AAU6 Q9QWY8 Q9ULH1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated peaks