BMRB Entry 11141

Name: Solution structure of the CH domain from human Vav-3 protein
Published: 2011-04-01

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PDB ID: 2d86
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11141
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CH domain from human Vav-3 protein

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain from human Vav-3 protein" .

Assembly members:

Assembly members:
CH domain, polymer, 143 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050404-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGMEPWKQCAQWLIH CKVLPTNHRVTWDSAQVFDL AQTLRDGVLLCQLLNNLRAH SINLKEINLRPQMSQFLCLK NIRTFLTACCETFGMRKSEL FEAFDLFDVRDFGKVIETLS RLSRTPIALATGIRPFPSGP SSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	635
15N chemical shifts	140
1H chemical shifts	1009

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CH domain	1

Entities:

Entity 1, CH domain 143 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

MET

GLU

PRO

2

TRP

LYS

GLN

CYS

ALA

GLN

TRP

LEU

ILE

HIS

3

CYS

LYS

VAL

LEU

PRO

THR

ASN

HIS

ARG

VAL

4

THR

TRP

ASP

SER

ALA

GLN

VAL

PHE

ASP

LEU

5

ALA

GLN

THR

LEU

ARG

ASP

GLY

VAL

LEU

6

CYS

GLN

LEU

ASN

LEU

ARG

ALA

HIS

7

SER

ILE

ASN

LEU

LYS

GLU

ILE

ASN

LEU

ARG

8

PRO

GLN

MET

SER

GLN

PHE

LEU

CYS

LEU

LYS

9

ASN

ILE

ARG

THR

PHE

LEU

THR

ALA

CYS

10

GLU

THR

PHE

GLY

MET

ARG

LYS

SER

GLU

LEU

11

PHE

GLU

ALA

PHE

ASP

LEU

PHE

ASP

VAL

ARG

12

ASP

PHE

GLY

LYS

VAL

ILE

GLU

THR

LEU

SER

13

ARG

LEU

SER

ARG

THR

PRO

ILE

ALA

LEU

ALA

14

THR

GLY

ILE

ARG

PRO

PHE

PRO

SER

GLY

PRO

15

SER

GLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.23 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2D86
DBJ	BAI45437
GB	AAC79695 AAD20349 AAI43970 AAI46366 AAI56727
REF	NP_006104 XP_001083337 XP_002801727 XP_002801728 XP_002928351
SP	Q9UKW4
AlphaFold	Q9UKW4