BMRB Entry 11140

Name: Solution structure of the 4th KH type I domain from human Vigilin
Published: 2011-04-01

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PDB ID: 2ctf
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11140
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 4th KH type I domain from human Vigilin

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 4th KH type I domain from human Vigilin" .

Assembly members:

Assembly members:
KH domain, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050131-13

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KH domain: GSSGSSGEPEKLGQALTEVY AKANSFTVSSVAAPSWLHRF IIGKKGQNLAKITQQMPKVH IEFTEGEDKITLEGPTEDVS VAQEQIEGMVKDLINRSGPS SG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	425
15N chemical shifts	98
1H chemical shifts	681

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KH domain	1

Entities:

Entity 1, KH domain 102 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

PRO

GLU

2

LYS

LEU

GLY

GLN

ALA

LEU

THR

GLU

VAL

TYR

3

ALA

LYS

ALA

ASN

SER

PHE

THR

VAL

SER

4

VAL

ALA

PRO

SER

TRP

LEU

HIS

ARG

PHE

5

ILE

GLY

LYS

GLY

GLN

ASN

LEU

ALA

6

LYS

ILE

THR

GLN

MET

PRO

LYS

VAL

HIS

7

ILE

GLU

PHE

THR

GLU

GLY

GLU

ASP

LYS

ILE

8

THR

LEU

GLU

GLY

PRO

THR

GLU

ASP

VAL

SER

9

VAL

ALA

GLN

GLU

GLN

ILE

GLU

GLY

MET

VAL

10

LYS

ASP

LEU

ILE

ASN

ARG

SER

GLY

PRO

SER

11

SER

GLY

Samples:

sample_1: KH domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2CTF
DBJ	BAC33456
EMBL	CAI46262
GB	EDL39954 EDL91932 EDL91937 EDL91944