BMRB Entry 11138

Name: Solution structure of the 13th KH type I domain from human Vigilin
Published: 2011-04-01

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PDB ID: 2ctl
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11138
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 13th KH type I domain from human Vigilin

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 13th KH type I domain from human Vigilin" .

Assembly members:

Assembly members:
KH domain, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050131-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KH domain: GSSGSSGEQEDRALRSFKLS VTVDPKYHPKIIGRKGAVIT QIRLEHDVNIQFPDKDDGNQ PQDQITITGYEKNTEAARDA ILRIVGELEQMSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	398
15N chemical shifts	95
1H chemical shifts	636

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KH domain	1

Entities:

Entity 1, KH domain 97 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

GLN

GLU

2

ASP

ARG

ALA

LEU

ARG

SER

PHE

LYS

LEU

SER

3

VAL

THR

VAL

ASP

PRO

LYS

TYR

HIS

PRO

LYS

4

ILE

GLY

ARG

LYS

GLY

ALA

VAL

ILE

THR

5

GLN

ILE

ARG

LEU

GLU

HIS

ASP

VAL

ASN

ILE

6

GLN

PHE

PRO

ASP

LYS

ASP

GLY

ASN

GLN

7

PRO

GLN

ASP

GLN

ILE

THR

ILE

THR

GLY

TYR

8

GLU

LYS

ASN

THR

GLU

ALA

ARG

ASP

ALA

9

ILE

LEU

ARG

ILE

VAL

GLY

GLU

LEU

GLU

GLN

10

MET

SER

GLY

PRO

SER

GLY

Samples:

sample_1: KH domain, [U-13C; U-15N], 1.38 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz