BMRB Entry 11134

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PDB ID: 2d7l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11134
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the HMG box domain from human WD repeat and HMG-box DNA binding protein 1

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.. "Solution structure of the HMG box domain from human WD repeat and HMG-box DNA binding protein 1" .

Assembly members:

Assembly members:
HMG box domain, polymer, 81 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050111-08

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HMG box domain: GSSGSSGRPKTGFQMWLEEN RSNILSDNPDFSDEADIIKE GMIRFRVLSTEERKVWANKA KGETASEGTEAKKRKSGPSS G

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	298
15N chemical shifts	76
1H chemical shifts	490

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HMG box domain	1

Entities:

Entity 1, HMG box domain 81 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

PRO

LYS

2

THR

GLY

PHE

GLN

MET

TRP

LEU

GLU

ASN

3

ARG

SER

ASN

ILE

LEU

SER

ASP

ASN

PRO

ASP

4

PHE

SER

ASP

GLU

ALA

ASP

ILE

LYS

GLU

5

GLY

MET

ILE

ARG

PHE

ARG

VAL

LEU

SER

THR

6

GLU

ARG

LYS

VAL

TRP

ALA

ASN

LYS

ALA

7

LYS

GLY

GLU

THR

ALA

SER

GLU

GLY

THR

GLU

8

ALA

LYS

ARG

LYS

SER

GLY

PRO

SER

9

GLY

Samples:

sample_1: HMG box domain, [U-13C; U-15N], 1.13 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2D7L
DBJ	BAF85694 BAG50934 BAG50944 BAG50951
EMBL	CAA06932
GB	AAH63041 EAW80654
REF	NP_001008397 NP_009017 XP_001161792 XP_003831741 XP_004055246
SP	O75717
AlphaFold	O75717