BMRB Entry 11133

Name: Solution structure of the fourth CH domain from human L-plastin
Published: 2011-04-01

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PDB ID: 2d85
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11133
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the fourth CH domain from human L-plastin

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the fourth CH domain from human L-plastin" .

Assembly members:

Assembly members:
CH domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050111-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGNDDIIVNWVNETL REAEKSSSISSFKDPKISTS LPVLDLIDAIQPGSINYDLL KTENLNDDEKLNNAKYAISM ARKIGARVYALPEDLVEVNP KMVMTVFACLMGKGMKRVSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	497
15N chemical shifts	117
1H chemical shifts	806

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CH domain	1

Entities:

Entity 1, CH domain 124 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

ASP

2

ILE

VAL

ASN

TRP

VAL

ASN

GLU

THR

LEU

3

ARG

GLU

ALA

GLU

LYS

SER

ILE

SER

4

SER

PHE

LYS

ASP

PRO

LYS

ILE

SER

THR

SER

5

LEU

PRO

VAL

LEU

ASP

LEU

ILE

ASP

ALA

ILE

6

GLN

PRO

GLY

SER

ILE

ASN

TYR

ASP

LEU

7

LYS

THR

GLU

ASN

LEU

ASN

ASP

GLU

LYS

8

LEU

ASN

ALA

LYS

TYR

ALA

ILE

SER

MET

9

ALA

ARG

LYS

ILE

GLY

ALA

ARG

VAL

TYR

ALA

10

LEU

PRO

GLU

ASP

LEU

VAL

GLU

VAL

ASN

PRO

11

LYS

MET

VAL

MET

THR

VAL

PHE

ALA

CYS

LEU

12

MET

GLY

LYS

GLY

MET

LYS

ARG

VAL

SER

GLY

13

PRO

SER

GLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2D85
DBJ	BAD97025 BAG35310
GB	AAA63236 AAB02845 AAH07673 AAH10271 ABM83861
REF	XP_001157685 XP_001158161 XP_002824296 XP_002824297 XP_003270105