BMRB Entry 11132

Title:
Solution structure of the HMG box like domain from human hypothetical protein FLJ14904
Deposition date:
2010-03-31
Original release date:
2011-04-01
Authors:
Tomizawa, T.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Kigawa, T.; Sato, M.; Koshiba, S.; Inoue, M.; Kamatari, Y.; Yokoyama, S.. "Solution structure of the HMG box like domain from human hypothetical protein FLJ14904"  .

Assembly members:

Assembly members:
HMG box like domain, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050125-12

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts80
1H chemical shifts592

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HMG box like domain1

Entities:

Entity 1, HMG box like domain 93 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETPROASN
2   ARGLYSALASERARGASNALATYRTYRPHE
3   PHEVALGLNGLULYSILEPROGLULEUARG
4   ARGARGGLYLEUPROVALALAARGVALALA
5   ASPALAILEPROTYRCYSSERSERASPTRP
6   ALALEULEUARGGLUGLUGLULYSGLULYS
7   TYRALAGLUMETALAARGGLUTRPARGALA
8   ALAGLNGLYLYSASPPROGLYPROSERGLU
9   LYSGLNLYSPROVALPHETHRSERGLYPRO
10   SERSERGLY

Samples:

sample_1: HMG box like domain, [U-13C; U-15N], 1.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB55385 BAE01036 BAJ20777
GB AAH28595 AAY82463 ADQ32386 AIC52639 EAW90780
REF NP_001247890 NP_116247 XP_002809887 XP_003785786 XP_003893548
SP Q4R786 Q96JY0
AlphaFold Q4R786 Q96JY0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks