BMRB Entry 11131

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PDB ID: 1x4u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11131
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the FYVE domain from human FYVE domain containing 27 isoform b protein

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the FYVE domain from human FYVE domain containing 27 isoform b protein" .

Assembly members:

Assembly members:
FYVE domain, polymer, 84 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041206-02

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FYVE domain: GSSGSSGRYPTNNFGNCTGC SATFSVLKKRRSCSNCGNSF CSRCCSFKVPKSSMGATAPE AQRETVFVCASCNQTLSKSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	311
15N chemical shifts	76
1H chemical shifts	467

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FYVE domain	1
2	ZINC ION no.1	2
3	ZINC ION no.2	2

Entities:

Entity 1, FYVE domain 84 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

TYR

PRO

2

THR

ASN

PHE

GLY

ASN

CYS

THR

GLY

CYS

3

SER

ALA

THR

PHE

SER

VAL

LEU

LYS

ARG

4

ARG

SER

CYS

SER

ASN

CYS

GLY

ASN

SER

PHE

5

CYS

SER

ARG

CYS

SER

PHE

LYS

VAL

PRO

6

LYS

SER

MET

GLY

ALA

THR

ALA

PRO

GLU

7

ALA

GLN

ARG

GLU

THR

VAL

PHE

VAL

CYS

ALA

8

SER

CYS

ASN

GLN

THR

LEU

SER

LYS

SER

GLY

9

PRO

SER

GLY

Entity 2, ZINC ION no.1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: zf-AN1 domain, [U-13C; U-15N], 1.83 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; ZnCl2 0.25 mM; IDA 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1X4U
DBJ	BAC05200 BAC11260 BAH12306 BAH12390 BAH13112
EMBL	CAD38913 CAH92258
GB	AAH30621 AAH61741 AIC61199 AKI69883 EAW49895
REF	NP_001002261 NP_001002262 NP_001126322 NP_001167590 NP_001167591
SP	Q5R7K2 Q5T4F4 Q6P7B7
AlphaFold	Q5R7K2 Q5T4F4 Q6P7B7