BMRB Entry 11129

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PDB ID: 1wyr
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11129
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CH domain of human Rho guanine nucleotide exchange factor 6

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain of human Rho guanine nucleotide exchange factor 6" .

Assembly members:

Assembly members:
CH domain, polymer, 121 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040910-16

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGEEQIVTWLISLGV LESPKKTICDPEEFLKSSLK NGVVLCKLINRLMPGSVEKF CLDPQTEADCINNINDFLKG CATLQVEIFDPDDLYSGVNF SKVLSTLLAVNKATESGPSS G

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	509
15N chemical shifts	117
1H chemical shifts	807

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CH domain	1

Entities:

Entity 1, CH domain 121 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

GLN

2

ILE

VAL

THR

TRP

LEU

ILE

SER

LEU

GLY

VAL

3

LEU

GLU

SER

PRO

LYS

THR

ILE

CYS

ASP

4

PRO

GLU

PHE

LEU

LYS

SER

LEU

LYS

5

ASN

GLY

VAL

LEU

CYS

LYS

LEU

ILE

ASN

6

ARG

LEU

MET

PRO

GLY

SER

VAL

GLU

LYS

PHE

7

CYS

LEU

ASP

PRO

GLN

THR

GLU

ALA

ASP

CYS

8

ILE

ASN

ILE

ASN

ASP

PHE

LEU

LYS

GLY

9

CYS

ALA

THR

LEU

GLN

VAL

GLU

ILE

PHE

ASP

10

PRO

ASP

LEU

TYR

SER

GLY

VAL

ASN

PHE

11

SER

LYS

VAL

LEU

SER

THR

LEU

ALA

VAL

12

ASN

LYS

ALA

THR

GLU

SER

GLY

PRO

SER

13

GLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.32 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WYR
DBJ	BAF84431
EMBL	CAD97632
GB	AAH39856 EAW88460 EHH31126 EHH61270
REF	NP_001253502 NP_004831 XP_002763367 XP_002832212 XP_003272624
SP	Q15052
AlphaFold	Q15052