BMRB Entry 11125

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PDB ID: 1x4x
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11125
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 6th fibronectin type III domain from human fibronectin type III domain containing protein 3

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the 6th fibronectin type III domain from human fibronectin type III domain containing protein 3" .

Assembly members:

Assembly members:
fibronectin type III (fn3) domain, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040705-15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fibronectin type III (fn3) domain: GSSGSSGPDQCKPPQVTCRS ATCAQVNWEVPLSNGTDVTE YRLEWGGVEGSMQICYCGPG LSYEIKGLSPATTYYCRVQA LSVVGAGPFSEVVACVTPPS SGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	404
15N chemical shifts	96
1H chemical shifts	637

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	fibronectin type III (fn3) domain	1

Entities:

Entity 1, fibronectin type III (fn3) domain 106 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

ASP

GLN

2

CYS

LYS

PRO

GLN

VAL

THR

CYS

ARG

SER

3

ALA

THR

CYS

ALA

GLN

VAL

ASN

TRP

GLU

VAL

4

PRO

LEU

SER

ASN

GLY

THR

ASP

VAL

THR

GLU

5

TYR

ARG

LEU

GLU

TRP

GLY

VAL

GLU

GLY

6

SER

MET

GLN

ILE

CYS

TYR

CYS

GLY

PRO

GLY

7

LEU

SER

TYR

GLU

ILE

LYS

GLY

LEU

SER

PRO

8

ALA

THR

TYR

CYS

ARG

VAL

GLN

ALA

9

LEU

SER

VAL

GLY

ALA

GLY

PRO

PHE

SER

10

GLU

VAL

ALA

CYS

VAL

THR

PRO

SER

11

SER

GLY

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 0.66 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz