BMRB Entry 11124

Name: Solution structure of the CH domain of human Calponin 1
Published: 2011-04-01

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PDB ID: 1wyp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11124
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the CH domain of human Calponin 1

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the CH domain of human Calponin 1" .

Assembly members:

Assembly members:
CH domain, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040621-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGNKLAQKYDHQREQ ELREWIEGVTGRRIGNNFMD GLKDGIILCEFINKLQPGSV KKINESTQNWHQLENIGNFI KAITKYGVKPHDIFEANDLF ENTNHTQVQSTLLALASMAK TKGNKVNVGVSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	565
15N chemical shifts	144
1H chemical shifts	912

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CH domain	1

Entities:

Entity 1, CH domain 136 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

LYS

LEU

2

ALA

GLN

LYS

TYR

ASP

HIS

GLN

ARG

GLU

GLN

3

GLU

LEU

ARG

GLU

TRP

ILE

GLU

GLY

VAL

THR

4

GLY

ARG

ILE

GLY

ASN

PHE

MET

ASP

5

GLY

LEU

LYS

ASP

GLY

ILE

LEU

CYS

GLU

6

PHE

ILE

ASN

LYS

LEU

GLN

PRO

GLY

SER

VAL

7

LYS

ILE

ASN

GLU

SER

THR

GLN

ASN

TRP

8

HIS

GLN

LEU

GLU

ASN

ILE

GLY

ASN

PHE

ILE

9

LYS

ALA

ILE

THR

LYS

TYR

GLY

VAL

LYS

PRO

10

HIS

ASP

ILE

PHE

GLU

ALA

ASN

ASP

LEU

PHE

11

GLU

ASN

THR

ASN

HIS

THR

GLN

VAL

GLN

SER

12

THR

LEU

ALA

LEU

ALA

SER

MET

ALA

LYS

13

THR

LYS

GLY

ASN

LYS

VAL

ASN

VAL

GLY

VAL

14

SER

GLY

PRO

SER

GLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.10 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WYP
DBJ	BAA12983 BAG62488 BAH13577 BAH14777 BAK61945
GB	AAF18235 AAN73252 EAW84228 EDL25254 EDL78233
REF	NP_001267033 NP_001295270 NP_001295271 XP_003275805 XP_004060093