BMRB Entry 11120

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PDB ID: 1x5h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11120
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of the third fibronectin type III domain of human Neogenin

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the third fibronectin type III domain of human Neogenin" .

Assembly members:

Assembly members:
fn3 domain, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041213-04

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fn3 domain: GSSGSSGDVAVRTLSDVPSA APQNLSLEVRNSKSIMIHWQ PPAPATQNGQITGYKIRYRK ASRKSDVTETLVSGTQLSQL IEGLDRGTEYNFRVAALTIN GTGPATDWLSAETFESDLDE TRVPEVSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	531
15N chemical shifts	130
1H chemical shifts	835

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	fn3 domain	1

Entities:

Entity 1, fn3 domain 132 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASP

VAL

ALA

2

VAL

ARG

THR

LEU

SER

ASP

VAL

PRO

SER

ALA

3

ALA

PRO

GLN

ASN

LEU

SER

LEU

GLU

VAL

ARG

4

ASN

SER

LYS

SER

ILE

MET

ILE

HIS

TRP

GLN

5

PRO

ALA

PRO

ALA

THR

GLN

ASN

GLY

GLN

6

ILE

THR

GLY

TYR

LYS

ILE

ARG

TYR

ARG

LYS

7

ALA

SER

ARG

LYS

SER

ASP

VAL

THR

GLU

THR

8

LEU

VAL

SER

GLY

THR

GLN

LEU

SER

GLN

LEU

9

ILE

GLU

GLY

LEU

ASP

ARG

GLY

THR

GLU

TYR

10

ASN

PHE

ARG

VAL

ALA

LEU

THR

ILE

ASN

11

GLY

THR

GLY

PRO

ALA

THR

ASP

TRP

LEU

SER

12

ALA

GLU

THR

PHE

GLU

SER

ASP

LEU

ASP

GLU

13

THR

ARG

VAL

PRO

GLU

VAL

SER

GLY

PRO

SER

14

SER

GLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.5 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz