BMRB Entry 11118

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PDB ID: 1x5j
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11118
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of the fifth fibronectin type III domain of human Neogenin

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the fifth fibronectin type III domain of human Neogenin" .

Assembly members:

Assembly members:
fn3 domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041213-06

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fn3 domain: GSSGSSGPMMPPVGVQASIL SHDTIRITWADNSLPKHQKI TDSRYYTVRWKTNIPANTKY KNANATTLSYLVTGLKPNTL YEFSVMVTKGRRSSTWSMTA HGTTFELSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	471
15N chemical shifts	102
1H chemical shifts	725

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	fn3 domain	1

Entities:

Entity 1, fn3 domain 113 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

MET

2

PRO

VAL

GLY

VAL

GLN

ALA

SER

ILE

LEU

3

SER

HIS

ASP

THR

ILE

ARG

ILE

THR

TRP

ALA

4

ASP

ASN

SER

LEU

PRO

LYS

HIS

GLN

LYS

ILE

5

THR

ASP

SER

ARG

TYR

THR

VAL

ARG

TRP

6

LYS

THR

ASN

ILE

PRO

ALA

ASN

THR

LYS

TYR

7

LYS

ASN

ALA

ASN

ALA

THR

LEU

SER

TYR

8

LEU

VAL

THR

GLY

LEU

LYS

PRO

ASN

THR

LEU

9

TYR

GLU

PHE

SER

VAL

MET

VAL

THR

LYS

GLY

10

ARG

SER

THR

TRP

SER

MET

THR

ALA

11

HIS

GLY

THR

PHE

GLU

LEU

SER

GLY

PRO

12

SER

GLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.3 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	1X5J 3P4L 4BQ6 4BQ7 4BQ8 4BQ9 4BQB 4BQC 4PLN 4UI2
DBJ	BAD92649 BAI46643
EMBL	CAA70727
GB	AAB17263 AAB41100 AAC51287 AAC59662 AAH54540
PRF	2103267A
REF	NP_001036217 NP_001166094 NP_001166095 NP_001248429 NP_002490
SP	P97603 P97798 Q90610 Q92859
TPG	DAA25834
AlphaFold	P97603 P97798 Q90610 Q92859