BMRB Entry 11117

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PDB ID: 1x5a
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11117
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of the second fibronectin type III domain of mouse Ephrin type-A receptor 1

Deposition date:

2010-03-31

Original release date:

2011-04-01

Authors:

Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Sasagawa, A.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the second fibronectin type III domain of mouse Ephrin type-A receptor 1" .

Assembly members:

Assembly members:
Fibronectin type-III domain, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040719-10

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Fibronectin type-III domain: GSSGSSGAESLSGLSLKLVK KEPRQLELTWAGSRPRNPGG NLSYELHVLNQDEEWHQMVL EPRVLLTKLQPDTTYIVRVR TLTPLGPGPFSPDHEFRTSP PSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	445
15N chemical shifts	95
1H chemical shifts	692

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Fibronectin type-III domain	1

Entities:

Entity 1, Fibronectin type-III domain 107 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ALA

GLU

SER

2

LEU

SER

GLY

LEU

SER

LEU

LYS

LEU

VAL

LYS

3

LYS

GLU

PRO

ARG

GLN

LEU

GLU

LEU

THR

TRP

4

ALA

GLY

SER

ARG

PRO

ARG

ASN

PRO

GLY

5

ASN

LEU

SER

TYR

GLU

LEU

HIS

VAL

LEU

ASN

6

GLN

ASP

GLU

TRP

HIS

GLN

MET

VAL

LEU

7

GLU

PRO

ARG

VAL

LEU

THR

LYS

LEU

GLN

8

PRO

ASP

THR

TYR

ILE

VAL

ARG

VAL

ARG

9

THR

LEU

THR

PRO

LEU

GLY

PRO

GLY

PRO

PHE

10

SER

PRO

ASP

HIS

GLU

PHE

ARG

THR

SER

PRO

11

PRO

SER

GLY

PRO

SER

GLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.2 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1X5A
DBJ	BAC25971
GB	AAC52384 AAG12206 AAH71215 EDL13481 EDM15513
PRF	2207387A
REF	NP_001101328 NP_076069 XP_003504401 XP_005363880 XP_005405417
SP	Q60750
AlphaFold	Q60750