BMRB Entry 11112

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PDB ID: 1x5i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11112
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of the fourth fibronectin type III domain of human Neogenin

Deposition date:

2010-02-18

Original release date:

2011-02-18

Authors:

Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the fourth fibronectin type III domain of human Neogenin" .

Assembly members:

Assembly members:
fn3 domain, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041213-05

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fn3 domain: GSSGSSGPATDWLSAETFES DLDETRVPEVPSSLHVRPLV TSIVVSWTPPENQNIVVRGY AIGYGIGSPHAQTIKVDYKQ RYYTIENLDPSSHYVITLKA FNNVGEGIPLYESAVTRPHT SGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	511
15N chemical shifts	115
1H chemical shifts	790

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	fn3 domain	1

Entities:

Entity 1, fn3 domain 126 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

PRO

ALA

THR

2

ASP

TRP

LEU

SER

ALA

GLU

THR

PHE

GLU

SER

3

ASP

LEU

ASP

GLU

THR

ARG

VAL

PRO

GLU

VAL

4

PRO

SER

LEU

HIS

VAL

ARG

PRO

LEU

VAL

5

THR

SER

ILE

VAL

SER

TRP

THR

PRO

6

GLU

ASN

GLN

ASN

ILE

VAL

ARG

GLY

TYR

7

ALA

ILE

GLY

TYR

GLY

ILE

GLY

SER

PRO

HIS

8

ALA

GLN

THR

ILE

LYS

VAL

ASP

TYR

LYS

GLN

9

ARG

TYR

THR

ILE

GLU

ASN

LEU

ASP

PRO

10

SER

HIS

TYR

VAL

ILE

THR

LEU

LYS

ALA

11

PHE

ASN

VAL

GLY

GLU

GLY

ILE

PRO

LEU

12

TYR

GLU

SER

ALA

VAL

THR

ARG

PRO

HIS

THR

13

SER

GLY

PRO

SER

GLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.5 mM; d-Tris HCl, [U-2H], 20 mM; NaCl 100 mM; d-DTT, [U-2H], 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-SEPARATED NOESY	sample_1	isotropic	condition_1
3D 13C-SEPARATED NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 900 MHz

PDB	1X5I 4PLN
DBJ	BAD92649 BAI46643
EMBL	CAA70727
GB	AAB17263 AAB41100 AAC51287 AAH54540 AAI17162
REF	NP_001036217 NP_001166094 NP_001166095 NP_001248429 NP_002490
SP	P97603 P97798 Q92859
TPG	DAA25834
AlphaFold	P97603 P97798 Q92859