BMRB Entry 11106

Name: The solution structure of the FN3 domain of human Midline 2 protein
Published: 2011-02-18

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PDB ID: 2dmk
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11106
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The solution structure of the FN3 domain of human Midline 2 protein

Deposition date:

2010-02-18

Original release date:

2011-02-18

Authors:

Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the FN3 domain of human Midline 2 protein" .

Assembly members:

Assembly members:
fn3 domain, polymer, 127 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050302-86

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fn3 domain: GSSGSSGEGLDYLTAPNPPS IREELCTASHDTITVHWISD DEFSISSYELQYTIFTGQAN FISLYNSVDSWMIVPNIKQN HYTVHGLQSGTRYIFIVKAI NQAGSRNSEPTRLKTNSQPF KSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	539
15N chemical shifts	127
1H chemical shifts	822

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	fn3 domain	1

Entities:

Entity 1, fn3 domain 127 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

GLY

LEU

2

ASP

TYR

LEU

THR

ALA

PRO

ASN

PRO

SER

3

ILE

ARG

GLU

LEU

CYS

THR

ALA

SER

HIS

4

ASP

THR

ILE

THR

VAL

HIS

TRP

ILE

SER

ASP

5

ASP

GLU

PHE

SER

ILE

SER

TYR

GLU

LEU

6

GLN

TYR

THR

ILE

PHE

THR

GLY

GLN

ALA

ASN

7

PHE

ILE

SER

LEU

TYR

ASN

SER

VAL

ASP

SER

8

TRP

MET

ILE

VAL

PRO

ASN

ILE

LYS

GLN

ASN

9

HIS

TYR

THR

VAL

HIS

GLY

LEU

GLN

SER

GLY

10

THR

ARG

TYR

ILE

PHE

ILE

VAL

LYS

ALA

ILE

11

ASN

GLN

ALA

GLY

SER

ARG

ASN

SER

GLU

PRO

12

THR

ARG

LEU

LYS

THR

ASN

SER

GLN

PRO

PHE

13

LYS

SER

GLY

PRO

SER

GLY

Samples:

sample_1: fn3 domain, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-SEPARATED NOESY	sample_1	isotropic	condition_1
3D 13C-SEPARATED NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMNMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.955, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 700 MHz

PDB	2DMK
EMBL	CAB56170
GB	AAF07340 AAF07341 AAH17707 AAI40362 AAI46505
REF	NP_035975 NP_438112 XP_001091577 XP_001144794 XP_001491041
SP	Q9QUS6
TPG	DAA13074
AlphaFold	Q9QUS6