BMRB Entry 11103

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11103
MolProbity Validation Chart

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Title:
Solution structure of the second fibronectin type III domain of human Netrin receptor DCC
Deposition date:
2010-02-18
Original release date:
2011-02-17
Authors:
Tochio, N.; Koshiba, S.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Koshiba, S.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the second fibronectin type III domain of human Netrin receptor DCC"  .

Assembly members:

Assembly members:
second fn3 domain, Fibronectin type-III 2, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050905-21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
second fn3 domain, Fibronectin type-III 2: GSSGSSGPGPVENLQAVSTS PTSILITWEPPAYANGPVQG YRLFCTEVSTGKEQNIEVDG LSYKLEGLKKFTEYSLRFLA YNRYGPGVSTDDITVVTLSD SGPSSG

Data sets:
Data typeCount
13C chemical shifts434
15N chemical shifts98
1H chemical shifts671

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1second fn3 domain, Fibronectin type-III 21

Entities:

Entity 1, second fn3 domain, Fibronectin type-III 2 106 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROGLYPRO
2   VALGLUASNLEUGLNALAVALSERTHRSER
3   PROTHRSERILELEUILETHRTRPGLUPRO
4   PROALATYRALAASNGLYPROVALGLNGLY
5   TYRARGLEUPHECYSTHRGLUVALSERTHR
6   GLYLYSGLUGLNASNILEGLUVALASPGLY
7   LEUSERTYRLYSLEUGLUGLYLEULYSLYS
8   PHETHRGLUTYRSERLEUARGPHELEUALA
9   TYRASNARGTYRGLYPROGLYVALSERTHR
10   ASPASPILETHRVALVALTHRLEUSERASP
11   SERGLYPROSERSERGLY

Samples:

sample_1: second fn3 domain, Fibronectin type-III 2, [U-13C; U-15N], 1.2 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropiccondition_1
3D 1H-13C NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMNMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.955, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
REF XP_003914439

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks