BMRB Entry 11096

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11096
MolProbity Validation Chart

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Title:
Solution structure of the first fibronectin type III domain of human Netrin receptor DCC
Deposition date:
2010-02-19
Original release date:
2011-02-17
Authors:
Tochio, N.; Koshiba, S.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Koshiba, S.; Tomizawa, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the first fibronectin type III domain of human Netrin receptor DCC"  .

Assembly members:

Assembly members:
first fn3 domain, Fibronectin type-III 1, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050905-20

Entity Sequences (FASTA):

Entity Sequences (FASTA):
first fn3 domain, Fibronectin type-III 1: GSSGSSGKPAIPSSSVLPSA PRDVVPVLVSSRFVRLSWRP PAEAKGNIQTFTVFFSREGD NRERALNTTQPGSLQLTVGN LKPEAMYTFRVVAYNEWGPG ESSQPIKVATQPESGPSSG

Data sets:
Data typeCount
13C chemical shifts468
15N chemical shifts101
1H chemical shifts723

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1first fn3 domain, Fibronectin type-III 11

Entities:

Entity 1, first fn3 domain, Fibronectin type-III 1 119 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSPROALA
2   ILEPROSERSERSERVALLEUPROSERALA
3   PROARGASPVALVALPROVALLEUVALSER
4   SERARGPHEVALARGLEUSERTRPARGPRO
5   PROALAGLUALALYSGLYASNILEGLNTHR
6   PHETHRVALPHEPHESERARGGLUGLYASP
7   ASNARGGLUARGALALEUASNTHRTHRGLN
8   PROGLYSERLEUGLNLEUTHRVALGLYASN
9   LEULYSPROGLUALAMETTYRTHRPHEARG
10   VALVALALATYRASNGLUTRPGLYPROGLY
11   GLUSERSERGLNPROILELYSVALALATHR
12   GLNPROGLUSERGLYPROSERSERGLY

Samples:

sample_1: first fn3 domain, Fibronectin type-III 1, [U-13C; U-15N], 1.3 mM; d-Tris-HCl, [U-2H], 20 mM; sodium chloride 100 mM; d-DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropiccondition_1
3D 1H-13C NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMNMRView v5.0.4, Johnson, B.A - data analysis

Kujira v0.955, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

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