BMRB Entry 11094

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR11094
MolProbity Validation Chart

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Title:
Solution structure of the complex of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of hALK
Deposition date:
2010-02-15
Original release date:
2011-02-14
Authors:
Li, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the complex of the PTB domain of SNT-2 and 19-residue peptide (aa 1571-1589) of hALK"  .

Assembly members:

Assembly members:
PTB domain, polymer, 146 residues, Formula weight is not available
residues in database 1571-1589, polymer, 19 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060320-18

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PTB domain: GSSGSSGLNRDSVPDNHPTK FKVTNVDDEGVELGSGVMEL TQSELVLHLHRREAVRWPYL CLRRYGYDSNLFSFESGRRC QTGQGIFAFKCSRAEEIFNL LQDLMQCNSINVMEEPVIIT RNSHPAELDLPRAPQPPNAL GYTVSS
residues in database 1571-1589: LFRLRHFPCGNVNYGYQQQ

Data sets:
Data typeCount
13C chemical shifts610
15N chemical shifts141
1H chemical shifts1088

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PTB domain1
2residues in database 1571-15892

Entities:

Entity 1, PTB domain 146 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLEUASNARG
2   ASPSERVALPROASPASNHISPROTHRLYS
3   PHELYSVALTHRASNVALASPASPGLUGLY
4   VALGLULEUGLYSERGLYVALMETGLULEU
5   THRGLNSERGLULEUVALLEUHISLEUHIS
6   ARGARGGLUALAVALARGTRPPROTYRLEU
7   CYSLEUARGARGTYRGLYTYRASPSERASN
8   LEUPHESERPHEGLUSERGLYARGARGCYS
9   GLNTHRGLYGLNGLYILEPHEALAPHELYS
10   CYSSERARGALAGLUGLUILEPHEASNLEU
11   LEUGLNASPLEUMETGLNCYSASNSERILE
12   ASNVALMETGLUGLUPROVALILEILETHR
13   ARGASNSERHISPROALAGLULEUASPLEU
14   PROARGALAPROGLNPROPROASNALALEU
15   GLYTYRTHRVALSERSER

Entity 2, residues in database 1571-1589 19 residues - Formula weight is not available

1   LEUPHEARGLEUARGHISPHEPROCYSGLY
2   ASNVALASNTYRGLYTYRGLNGLNGLN

Samples:

sample_1: PTB domain, [U-13C; U-15N], 0.37 mM; hALK peptide 0.37 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropiccondition_1
3D 1H-13C NOESYsample_1isotropiccondition_1
3D 13C,15N-F1 FILTERED 13C-EDITED NOESYsample_1isotropiccondition_1
3D 13C,15N-F1 FILTERED 15N-EDITED NOESYsample_1isotropiccondition_1
2D F2-13C FILTERED NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

BMRB 11095 11095
PDB
DBJ BAJ20358 BAA08343 BAD92714 BAF73611 BAF73612 BAG10812
EMBL CAG33307
GB AAB92555 AAH10611 AAI46159 ABM83001 ABM86194 AAA58698 AAB71619 AAC51104 AAF27292 AAF42734
REF NP_001092388 NP_001162219 NP_001253120 NP_006644 XP_001501207 NP_001162572 NP_004295 NP_031465 XP_001092966 XP_001500618
SP O43559 P97793 Q9UM73
TPG DAA16527
AlphaFold O43559 P97793 Q9UM73

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks