BMRB Entry 11092

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11092
MolProbity Validation Chart

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Title:
Solution structure of the chromo domain of Mortality factor 4-like protein 1 from human
Deposition date:
2010-01-18
Original release date:
2011-01-19
Authors:
Li, H.; Sato, M.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Sato, M.; Tochio, N.; Tomizawa, T.; Koshiba, S.; Harada, T.; Watanabe, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the chromo domain of Mortality factor 4-like protein 1 from human"  .

Assembly members:

Assembly members:
Chromo domain, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: Cell free synthesis   Host organism: E. coli - cell free   Vector: P060522-07

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Chromo domain: GSSGSSGMAPKQDPKPKFQE GERVLCFHGPLLYEAKCVKV AIKDKQVKYFIHYSGWNKNW DEWVPESRVLKYVDTNLQKQ RELQKANQEQYAEGKMRGAA

Data sets:
Data typeCount
13C chemical shifts439
15N chemical shifts98
1H chemical shifts692

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Chromo domain1

Entities:

Entity 1, Chromo domain 100 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETALAPRO
2   LYSGLNASPPROLYSPROLYSPHEGLNGLU
3   GLYGLUARGVALLEUCYSPHEHISGLYPRO
4   LEULEUTYRGLUALALYSCYSVALLYSVAL
5   ALAILELYSASPLYSGLNVALLYSTYRPHE
6   ILEHISTYRSERGLYTRPASNLYSASNTRP
7   ASPGLUTRPVALPROGLUSERARGVALLEU
8   LYSTYRVALASPTHRASNLEUGLNLYSGLN
9   ARGGLULEUGLNLYSALAASNGLNGLUGLN
10   TYRALAGLUGLYLYSMETARGGLYALAALA

Samples:

sample_1: Chromo domain, [U-13C; U-15N], 1.13 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9827, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB30219 BAB58904 BAE00783 BAE26980 BAE27430
EMBL CAB70879 CAI29614
GB AAD20970 AAD29872 AAF29033 AAF80854 AAG17253
REF NP_001011999 NP_001030525 NP_001032250 NP_001032253 NP_001127679
SP Q5NVP9 Q6AYU1
TPG DAA17605
AlphaFold Q5NVP9 Q6AYU1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks