BMRB Entry 11089

Title:
Solution structure of the PH domain of Pleckstrin homology domain-containing family A member 6 from human
Deposition date:
2010-01-18
Original release date:
2011-01-19
Authors:
Li, H.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PH domain of Pleckstrin homology domain-containing family A member 6 from human"  .

Assembly members:

Assembly members:
PH domain, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: Cell free synthesis   Host organism: E. coli - cell free   Vector: P060515-01

Data sets:
Data typeCount
13C chemical shifts519
15N chemical shifts116
1H chemical shifts806

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PH domain1

Entities:

Entity 1, PH domain 122 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLYLYSARG
2   SERHISSERMETLYSARGASNPROASNALA
3   PROVALTHRLYSALAGLYTRPLEUPHELYS
4   GLNALASERSERGLYVALLYSGLNTRPASN
5   LYSARGTRPPHEVALLEUVALASPARGCYS
6   LEUPHETYRTYRLYSASPGLULYSGLUGLU
7   SERILELEUGLYSERILEPROLEULEUSER
8   PHEARGVALALAALAVALGLNPROSERASP
9   ASNILESERARGLYSHISTHRPHELYSALA
10   GLUHISALAGLYVALARGTHRTYRPHEPHE
11   SERALAGLUSERPROGLUGLUGLNGLUALA
12   TRPILEGLNALAMETGLYGLUALAALAARG
13   VALGLN

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.21 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA76813 BAG09923
GB AAH54547 AAI52476 AAI67845 EAW91509 EAW91510
REF NP_055750 NP_891846 XP_002808300 XP_002918242 XP_003474853
SP Q7TQG1 Q9Y2H5
AlphaFold Q7TQG1 Q9Y2H5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks