BMRB Entry 11089

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PDB ID: 2yry
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11089
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the PH domain of Pleckstrin homology domain-containing family A member 6 from human

Deposition date:

2010-01-18

Original release date:

2011-01-19

Authors:

Li, H.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Li, H.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PH domain of Pleckstrin homology domain-containing family A member 6 from human" .

Assembly members:

Assembly members:
PH domain, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Cell free synthesis Host organism: E. coli - cell free Vector: P060515-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PH domain: GSSGSSGGKRSHSMKRNPNA PVTKAGWLFKQASSGVKQWN KRWFVLVDRCLFYYKDEKEE SILGSIPLLSFRVAAVQPSD NISRKHTFKAEHAGVRTYFF SAESPEEQEAWIQAMGEAAR VQ

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	519
15N chemical shifts	116
1H chemical shifts	806

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PH domain	1

Entities:

Entity 1, PH domain 122 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

LYS

ARG

2

SER

HIS

SER

MET

LYS

ARG

ASN

PRO

ASN

ALA

3

PRO

VAL

THR

LYS

ALA

GLY

TRP

LEU

PHE

LYS

4

GLN

ALA

SER

GLY

VAL

LYS

GLN

TRP

ASN

5

LYS

ARG

TRP

PHE

VAL

LEU

VAL

ASP

ARG

CYS

6

LEU

PHE

TYR

LYS

ASP

GLU

LYS

GLU

7

SER

ILE

LEU

GLY

SER

ILE

PRO

LEU

SER

8

PHE

ARG

VAL

ALA

VAL

GLN

PRO

SER

ASP

9

ASN

ILE

SER

ARG

LYS

HIS

THR

PHE

LYS

ALA

10

GLU

HIS

ALA

GLY

VAL

ARG

THR

TYR

PHE

11

SER

ALA

GLU

SER

PRO

GLU

GLN

GLU

ALA

12

TRP

ILE

GLN

ALA

MET

GLY

GLU

ALA

ARG

13

VAL

GLN

Samples:

sample_1: PH domain, [U-13C; U-15N], 1.21 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	sample_1	isotropic	condition_1
3D 13C-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2YRY
DBJ	BAA76813 BAG09923
GB	AAH54547 AAI52476 AAI67845 EAW91509 EAW91510
REF	NP_055750 NP_891846 XP_002808300 XP_002918242 XP_003474853
SP	Q7TQG1 Q9Y2H5
AlphaFold	Q7TQG1 Q9Y2H5